Literature summary for 2.4.1.155 extracted from

Huang, X.; Liu, T.; Wang, Q.; Zhu, W.; Meng, H.; Guo, L.; Wei, T.; Zhang, J.
Inhibition of N-acetylglucosaminyltransferase V enhances the cetuximab-induced radiosensitivity of nasopharyngeal carcinoma cells likely through EGFR N-glycan alterations (2017), Glycobiology, 27, 713-725 .

Search for more literature for 2.4.1.155

Application

Application	Comment	Organism
medicine	radiotherapy combining GnT-V down-regulation and cetuximab decelerates tumor growth	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene MGAT5, recombinant expression of enzyme Gnt-V in CNE-1 and CNE-2 cells using the lentiviral transfection method	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	GnT-V is used as a molecular target to further sensitize cetuximab-treated nasopharyngeal carcinoma (NPC) cells to radiation. Development of GnT-V-suppressed cell models, denoted CNE1-1564, CNE1-2224, CNE2-1564 and CNE2-2224. Lentivirus-mediated shRNA inhibits GnT-V mRNA and protein expression in NPC cell lines. The expression levels of GnTV-V mRNA in these cell models were 36.3%, 33.9%, 42% and 35.5% of the levels found in the control groups, respectively. Similarly, the GnT-V protein levels in the CNE1-1564, CNE1-2224, CNE2-1564 and CNE2-2224 cell models are decreased by 60.13%, 59.89%, 43.67% and 46.18%, respectively. GnT-V expression and depletion of GnT-V in nasopharyngeal carcinoma cells, phenotypes, overview. Changes in the radiosensitivity of CNE-1 and CNE-2 cells are also related to PI3K/Akt signaling. The total Akt level does not vary among the different groups, but the phospho-Akt and phospho-PI3K levels present differences: the phospho-Akt and phospho-PI3K levels in CNE2-1564 and CNE2-2224 cells are lower than those in CNE-2-NC, and the levels detected in CNE2-1564/cetuximab and CNE2-2224/cetuximab are lower than those found in CNE2-1564 and CNE2-2224 cells. CNE2-NC/cetuximab cells present reduced levels of phospho-Akt and phospho-PI3K compared with CNE2-NC cells. The CNE-1 cells present similar results. In brief, the irradiation-induced alteration of beta-1,6 branches on the EGFR might influence PI3K/AKT signaling	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q09328	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
CNE-1 cell	-	Homo sapiens	-
CNE-2 cell	-	Homo sapiens	-
HNE-1 cell	-	Homo sapiens	-
additional information	CNE-2 cells express 7.5fold and 2.30fold higher levels of GnT-V mRNA and protein than HNE-1 cells, and CNE-1 cells express 1.41fold and 1.36fold higher levels of GnT-V mRNA and protein than HNE-1 cells	Homo sapiens	-
nasopharyngeal carcinoma cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
beta-1,6-N-acetylglucosaminyltransferase V	-	Homo sapiens
Mgat5	-	Homo sapiens
N-acetylglucosaminyltransferase V	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	inhibition of N-acetylglucosaminyltransferase V enhances the cetuximab-induced radiosensitivity of nasopharyngeal carcinoma (NPC) cells likely through EGFR N-glycan alterations. Cetuximab is an epidermal growth factor receptor (EGFR) inhibitor used as a radiosensitizer in the treatment of NPC. The half-maximal inhibitory concentration (IC50) of cetuximab in CNE-1 and CNE-2 cells is 0.717 and 1.244 mg/ml, respectively	Homo sapiens
physiological function	N-acetylglucosaminyltransferase V (GnT-V) catalyzes the formation of the N-linked beta-1-6 branching of oligosaccharides adding GlcNAc to beta-1,6-linked branches	Homo sapiens

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Release 2023.1 (January 2023)